Josie Rourke is an English theatre and film director. She is a Vice-President of the London Library and was the artistic director of the Donmar Warehouse theatre from 2012 to 2019. In 2018, she made her feature film debut with the Academy Award and BAFTA-nominated historical drama Mary Queen of Scots, starring Saoirse Ronan and Margot Robbie. During the 2020 lockdown, she directed the episode "Her Big Chance" of the BBC reboot of Alan Bennett's classic 1980s monologues, Talking Heads, starring Jodie Comer.

In summer 2021, she directed and co-wrote the star-studded short film Rhythm of Life, encouraging people to get COVID-19 vaccines. It featured the song "The Rhythm of Life" from the 1966 classic musical, Sweet Charity, and starred Jim Broadbent, Derek Jacobi, David Walliams, Asa Butterfield, Colin Salmon, Don Warrington, Nicola Roberts, Russel Tovey and West End dancers.